Analysis of self-assembled monolayers by thermal desorption mass spectrometry: neighborhood interaction and hydrogen/deuterium exchange.

On-tissue Direct Monitoring of Global Hydrogen/Deuterium Exchange by MALDI Mass Spectrometry: Tissue Deuterium Exchange Mass Spectrometry (TDXMS).

Hydrogen/deuterium exchange mass spectrometric (H/DXMS) methods for protein structural analysis are conventionally performed in solution. We present Tissue Deuterium Exchange Mass Spectrometry (TDXMS), a method to directly monitor deuterium uptake on tissue, as a means to better approximate the deuterium exchange behavior of proteins in their native microenvironment. Using this method, a differ...

Application of Hydrogen/Deuterium Exchange Mass Spectrometry in Structural Biology and Molecular Medicine

Studying the Conformational and Functional Impact of Chemical Degradations in the Antibody Complementarity-Determining Regions Using Hydrogen Deuterium Exchange Mass Spectrometry and ELISA

Desorption of neutral molecules from self-assembled monolayers subjected to keV ion bombardment

Laser postionization in combination with mass spectrometry was used to detect neutral molecules desorbed from self-assembled monolayers of phenethyl mercaptan and benzyl mercaptan on gold upon 8 keV Ar bombardment. From experimentally obtained time distributions, it was determined that the majority of the desorbed neutral molecules leave the surface with low translational energies of about 0.03...

Profiling Kinase Activities by Using a Peptide Chip and Mass SpectrometryThis work was supported by the National Science Foundation. D.-H.M. was supported by the Burroughs Wellcome Fund Interfaces Program.

Herein we describe a strategy that combines peptide chips with matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) to evaluate kinase activities rapidly and semi-quantitatively. We have already shown that monolayers of alkanethiolates on gold are wellsuited as substrates for MALDI-TOF MS, and could be used to measure enzyme activities and to perform hig...